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1/20/2023 0 Comments Pixel led panel![]() The long awaited BME680 from Bosch gives you all the environmental sensing you want in one small package. Operating System: Windows / macOS / Linuxįile Type: JPG / PNG / SVG / DXF / AI / PDF Mode: Vector / Graphic (monochrome, gray scale) I/O: Wi-Fi / USB/ Ethernet / Direct Network CableĬutting Thickness: 10+ mm (varies by material) HEXA 1.5 inch Focus Lens Add-on allows you to engrave up to 1,000 DPI. ![]() ![]() 10,000 hours of laser tube lifespan with 1 year warranty.Įquipped with electric elevation platform to easily focus with the tap of your fingers.ġ000 dpi resolution: HEXA 1.5 inch Focus Lens Add-on allows you to engrave up to 1,000 DPI. HEXA provides maximum engraving speed of 900mm/s, to fulfill business and professional requirements.Ħ0W High Spec Laser Tube: Brand new high spec 60W laser tube with optional 1.5 inch focus lens substantially shrinking laser spot, achieving 1000 DPI resolution. Built for more capacity, more possibilities.ĩ00 mm/s High Speed Engraving: Carrying the Cortex M4 core chip. FLUX HEXA brings your work to the next-level quality. With a huge working size of 730 x 410mm, FLUX HEXA is ready for bigger challenges.ģ3 % Bigger Working Area: With 730 x 410 x 125mm of working area, it’s 33% larger than Beambox Pro. do NOT fold this matrix in half!Įxceptional Performance with the Biggest Upgrade Ever. Do not bend this Panel too rough, you may damage LED traces or the panel itself. Note: Over bending may significantly reduce the structural integrity of the flexible panel. Needless to say, if you are looking for a large panel of addressable LEDs to fit the contours of almost any surface you’ve come to the right place. Attached to each flexible LED matrix are two 3-pin JST SM connectors and two sets of bare power cables (input / output), which provide you connections for DC5V+, COM-, DAT, and GND. These panels require a 5V low voltage input for power which needs to be able to source a good amount of current – up to 5A in most uses (15A if all LEDs are set to bright white, which is NOT recommended). ![]() On top of all that, thanks to its flexible backing, this LED Matrix can be bent and bowed to fit onto almost any curvy surface. 64 or 256 individually addressable LEDs on a flexible panel - you can create animations, games, or even incorporate them into a fun e-textiles project. These WS2812B flexible LED matrix are a great way to add an impressive amount of color while still being able to individually control each LED. ![]() 1/20/2023 0 Comments Piperoll 123![]() ![]() Henry died on 1 December 1135 after a week of illness. The relationship between Henry and the couple became strained, and fighting broke out along the border with Anjou. In response to this, he declared his daughter Matilda his heir and married her to Geoffrey of Anjou. Henry took a second wife, Adeliza of Louvain, in the hope of having another son, but their marriage was childless. Henry's son William drowned in the White Ship disaster of 1120, throwing the royal succession into doubt. He supported the Cluniac order and played a major role in the selection of the senior clergy in England and Normandy. Henry encouraged ecclesiastical reform, but became embroiled in a serious dispute in 1101 with Archbishop Anselm of Canterbury, which was resolved through a compromise solution in 1105. Many of the officials who ran Henry's system were "new men" of obscure backgrounds, rather than from families of high status, who rose through the ranks as administrators. Normandy was also governed through a growing system of justices and an exchequer. ![]() In England, he drew on the existing Anglo-Saxon system of justice, local government and taxation, but also strengthened it with additional institutions, including the royal exchequer and itinerant justices. Following Henry's victory at the Battle of Brémule, a favourable peace settlement was agreed with Louis in 1120.Ĭonsidered by contemporaries to be a harsh but effective ruler, Henry skillfully manipulated the barons in England and Normandy. ![]() Henry's control of Normandy was challenged by Louis VI of France, Baldwin VII of Flanders and Fulk V of Anjou, who promoted the rival claims of Robert's son, William Clito, and supported a major rebellion in the Duchy between 11. Henry kept Robert imprisoned for the rest of his life. The peace was short-lived, and Henry invaded the Duchy of Normandy in 11, finally defeating Robert at the Battle of Tinchebray. Robert, who invaded from Normandy in 1101, disputed Henry's control of England this military campaign ended in a negotiated settlement that confirmed Henry as king. He married Matilda of Scotland and they had two surviving children, Empress Matilda and William Adelin he also had many illegitimate children by his many mistresses. Present at the place where his brother William died in a hunting accident in 1100, Henry seized the English throne, promising at his coronation to correct many of William's less popular policies. He gradually rebuilt his power base in the Cotentin and allied himself with William Rufus against Robert. He purchased the County of Cotentin in western Normandy from Robert, but his brothers deposed him in 1091. On William's death in 1087, Henry's elder brothers Robert Curthose and William Rufus inherited Normandy and England, respectively, but Henry was left landless. He was the fourth son of William the Conqueror and was educated in Latin and the liberal arts. 1068 – 1 December 1135), also known as Henry Beauclerc, was King of England from 1100 to his death in 1135. ![]() ![]() ![]() You may find this ID in the Install and Uninstall troubleshooter by pointing the cursor on the package. (Error code 1603)ĬONFIG_TEXT: MSI (09): Application. Though this issue is prevalent among Windows 10 users, it has also been reported on earlier operating systems as far back as Windows Vista. Contact your technical support group.įailed to install 'C:\ParallelsInstaller\cache\MSODBCSQL-WIN\thirdparty-windows\msodbcsql-13.1.msi': Fatal error during installation.
![]() ![]() If this is the case, temporarily disable them and check if messages arrive in your Inbox folder. Make sure there are no mail filters that delete incoming messages or move them to other folders. This is a filter that deletes or moves email messages You can delete messages from the client, but leave them in Yandex.Mail by configure the client using POP3, but we don't recommend this as messages might not sync correctly with the server. This is because your client uses the IMAP protocol, and the inbox in your client is synced with the service. Currently, I am using Windows 10 mail app for my. As soon as it is started for the first time, Thunderbird asks. User Query: Hi I am Denny, working in an MNC. Mozilla Thunderbird is still the most popular third-party mail management program on Windows. Besides, Thunderbird is also an email client developed by Mozilla Corporation. Or you can easily download the Windows 10 Mail app from Windows Store. ![]() If you use an email client and delete email in it, messages are removed from the service page as well. Windows 11 Mail is an email client application provided by Microsoft with every Windows 10 edition. Thunderbird is an open source tool so it’s great for anyone who wants. Mozilla Firefox fans have an easy to use solution for their email needs. Thunderbird was good but now junk as any Windows update means you have to reconfigure your email addresses and Passwords again. You should also make sure there aren't any unknown phone numbers on the Phone numbers page. Thunderbird is Mozilla’s award-winning email solution. If you are sure that only you have access to your mailbox, test your computer with an antivirus software, change the password and alternative address. The interface it offers is pleasant and allows you to quickly find any message, access the calendar, tasks and contacts. If more than 2000 actions were performed after the messages were deleted, data on the delete operation will not be displayed in the log. ![]() 1/19/2023 0 Comments Texmacs gmp medium![]() ![]() This thesis aims to explore these avenues by developing and applying advanced decisional tools that analyse the gene therapy supply chain systematically whilst capturing multiple stakeholder perspectives. Given the relative infancy of the sector, there is a strong drive towards adopting technologies that minimise costs and supply chain complexity. chimeric antigen receptor T-cell therapy – CAR T), hence the supply chain of these products is highly complex. Most of today’s gene therapy products are viral vector-based, typically relying on plasmid DNA supply for their production, and many are autologous ex vivo applications (e.g. Gene therapy products have tremendous therapeutic potential for indications such as cancer and even curative potential for some genetic diseases. We conclude that CCR5-Uco-hetTALEN transfected by mRNA electroporation facilitates specific, high-efficiency CCR5 gene-editing (30%–56%) and it is highly suited for clinical translation subject to further characterisation of off-target effects. Using single-cell HRMCA, we discovered high rates of TALEN-induced biallelic gene editing of CCR5, which translated in large numbers of CCR5-negative cells resistant to HIVenv-pseudotyped lentiviral vectors. We optimised in vitro mRNA production and showed that CCR5-on- and CCR2 off-target activities of CCR5-Uco-hetTALEN were limited to the first 72 and 24–48 h post-mRNA electroporation, respectively. ![]() Simultaneous cutting at CCR5 and CCR2 induced rearrangements, mainly 15-kb deletions between the cut sites, in up to 2% of T cells underlining the necessity to restrict TALEN expression. Notably, the CCR5Δ55–60 protein resulting from the 18-bp deletion was almost completely retained in the cytosol. ![]() Prevailing 18- and 10-bp deletions at the on-target site revealed microhomology-mediated end-joining as a major repair pathway. The novel variant, CCR5-Uco-hetTALEN features an obligatory heterodimeric Fok1-cleavage domain, which resulted in complete abrogation of off-target activity at previously found homodimeric as well as 7/8 in silico predicted, potential heterodimeric off-target sites, the only exception being highly homologous CCR2. Here we optimised our previously described CCR5-Uco-TALEN and its delivery by mRNA electroporation. Blue triangles indicate time points of sample collection on day 0 before and after activation, on day 3 prior to electroporation, on days 4–7 and after harvest on day 12.ĭisruption of the C-C-Chemokine-receptor-5 (CCR5) gene induces resistance towards CCR5-tropic HIV. During the whole process, the cell culture was regularly agitated to ensure sufficient gas support. Day 12: Harvest of cells in final formulation buffer. Medium and waste bags were exchanged during these days. Day 4–11: Expansion phase with feeding or media replacing steps with supplemented TexMACS GMP Medium. Day 3: Automated electroporation of cells in CliniMACS Electroporation Buffer with TALEN mRNA and incubation of cells at 32 ☌ for 24 h. CD4⁺ cells were separated after positive magnetic labelling with CliniMACS CD4 Reagent from fresh buffy coats and subsequently activated using MACS GMP T cell TransAct. Day 0: Installation of tubing sets (TS 520 + EP-2), integrity test and priming after attachment of supplemented CliniMACS PBS/EDTA (0.5 % human albumin) and TexMACS GMP Medium (MACS GMP Recombinant Human IL-7 and IL-15 and 3% human serum). Workflow of the automated T Cell Engineering (TCE) process and set up of the tubing sets at the CliniMACS ProdigyĬCR5-edited CD4⁺-T cells are manufactured during a 12-day process after single installation of a fused tubing set. ![]() In conclusion, transfection of primary T cells with CCR5-Uco-hetTALEN mRNA is readily scalable for GMP-compatible production and hence suitable for application in HIV gene therapy. Of note, about 40% of total large-scale produced cells showed a biallelic CCR5 editing, and between 25 and 42% of produced cells had a central memory T-cell phenotype. The automated process reliably produced high amounts of CCR5-edited CD4+-T cells (>1.5 × 109 cells with >60% CCR5 editing) within 12 days. Here we present a GMP-compatible mRNA electroporation protocol for the automated production of CCR5-edited CD4+-T cells in the closed CliniMACS Prodigy system. Clinical translation of the knockout approach requires up-scaling of the manufacturing process to clinically relevant cell numbers in accordance with good manufacturing practice (GMP). To this end, we have developed and optimised a CCR5-targeting TALE nuclease, CCR5-Uco-hetTALEN, mediating high-efficiency knockout of C-C motif chemokine receptor 5 (CCR5), the HIV co-receptor essential during initial infection. One of those strategies is directed at the protection of CD4+-T helper cells from HIV infection in HIV-positive individuals. Ex-vivo gene editing in T lymphocytes paves the way for novel concepts of immunotherapy. ![]() 1/19/2023 0 Comments Ftl faster than light fanart![]() Well trained elite Mantis, who are faster and stronger than the lesser working class. The Mantis disregard for individual lives led to their evolution as a vicious warrior race.Įlite Mantis Crew. The extra damage also applies to weapon fire from the ship.Both modifiers (allDamageTaken and stunMultiplier) SET the values to x1.5 instead of modifying the base stat, this means that an Ampere under this effect is actually stunned for less time than it would without the effect.It will also be unable to man, repair or sabotage systems. The Cultivator will be immobile and deal half damage during the ability. Nearby enemies take x1.5 damage and are stunned for x1.5 duration. Active Ability (30 second cooldown, refills on jump): COMBUST - Release poisonous spores for 15 seconds.Passive Ability: Debuffs enemy crew in the same room by reducing their healing speed by x0.75.Refills oxygen very slowly (needs O2 system in the ship to work, +0.1 oxygen generation rate).These massive and terrifying Orchids are responsible for fueling the profitable spore trade, but also make deadly opponents. (currently can only be obtained via Promotion Bomb + Vampweed or starting with one in Wrecked Bomber C)Įlite Vampweed Crew. Debuffs enemy crew in the same room by reducing their Max HP by x0.85 and reducing their movement speed by x0.75.Refills oxygen slowly (needs O2 system in the ship to work, +0.25 oxygen generation rate).The Vampweeds, natively known as the Oro'Bache, are not known for the same friendliness as their unwelcome Floral brethren, but they do exhibit similar traits. Refills oxygen quickly (needs O2 system in the ship to work, +2 oxygen generation rate).Friendly crew in the same room gain 25% max health and 25% healing speed.Immune to suffocation makes allied crew in the same room immune to suffocation.Strong Orchids with a natural grown layer of wooden armor that protect the Orchid Garden-Worlds. Friendly crew in the same room gain 20% max health, 20% repair speed and 20% healing speed.Įlite Floral Crew.Refills oxygen (needs O2 system in the ship to work, +0.525 oxygen generation rate, same as a lanius/breach).The Florals, natively known as the Ctl'Yae, are a friendly species of mostly pacifist plants which can buff your crew and refill oxyen. will deal regular 15 death explosion damage if this is enough to kill them (friendlies take 30 hp damage, enemies 45 hp) Active Ability (8 second cooldown, resets on jump): Activates a 2s countdown, at which the end of, the martyr explodes, dealing 50 damage to themselves and dealing 30 damage and 3 seconds of stun to all crew in the room.Turns out, the devotees aren't the most dedicated to the Duskbringer ways. (8->6 and 18->14 respectively)Įlite Duskbringer crew. Learns combat and repair skills faster.Also deals regular 15 death explosion damage (friendlies take 30 hp damage, enemies 45 hp) Active Ability (8 second cooldown, resets on jump): Activates a 2s countdown, at which the end of, the duskbringer explodes, killing themselves and dealing 30 damage and 3 seconds of stun to all crew in the room.Deals 15 damage to enemy crew on death.The most devoted members of the Duskbringer Revolution, who are willing to take their own life in the name of their faith. Deals 30 damage to enemy crew on death and 1 point of ion damage to current system.Armored and trained Zoltan soldier who will gladly give you a kick in the teeth if you step out of line. Active Ability (27 second cooldown, which refills on jump): Stuns all crew in the room excluding the monk themselves for 10 seconds.Įlite Zoltan Crew.Deals 15 damage and 4 seconds of stun to enemy crew on death.Cannot fight crew or sabotage systems (allowing them to repair while enemies are in the room!).The heavily religious Zoltan monks live a life dedicated to non-violence. Their innate energy can power ship systems. These hard-light entities might be part of the Federation, but they're also known for their strict rules and formal attitudes. ![]() Active Ability (30 second cooldown, which refills on jump): Activates a shield for 15 seconds which sets damage taken from all sources to x0.1, makes the Defender immune to mind control, and allows the Defender to see adjacent rooms.A shielded Engi Soldier who protect Engi ships and sectors. Humans are common and uninteresting, but very skilled.Įlite Engi Crew. They have several sub-types that can be found in all kinds of sectors. Humans are the most common crew type in the game. ![]() 1/19/2023 0 Comments Quantum physics and spirituality![]() Theoretical physics does not deal with the question of ‘how’ this is decided, but only with the effervescent nature of the tiny energetic filaments which possess a dynamic potential to become anything. Within ‘string theory’ as with quantum mechanics, we once again discover a fluid and changeable reality that contains the property of ‘indeterminacy.’ What this means is that the tiny energy filaments that are known as ‘strings,’ (tinier than the tiniest sub-atomic particle that has ever been measured), can become anything that it is possible to become – a particle, a wave, a graviton, an electron, and so forth. This is six dimensions greater than the traditional model that we have typically held containing three dimensions of space and one dimension of time. Based on mathematical models that can be shared and refined but not yet observed directly within matter, ‘string theory’ predicts a physical world of ten dimensions, nine tiny curled-up spatial dimensions and one dimension of time. ![]() ‘String theory’ arose and became popular within physics as a way of responding to this wish. ‘String theory’ is the result of the efforts of theoretical physicists to complete the work of Albert Einstein in being able to articulate a unified field theory of physical reality that would apply to both the smallest sub-atomic aspects of that reality and to the larger spatial realities as well. Although its precursors arose in the nineteen-thirties with the great surge of interest in quantum mechanics and its research into the nature of the sub-atomic world, it was not until a mere twenty five years ago that the newer ‘string theory’ gave new meaning to and a new understanding of the fluid reality that we live in. This shift in our assumptions about physical matter is taking place now. But if something is energetically moving, it is fluid and changeable, depending upon where and how we view it. But what if nothing was solid in the physical universe, and all that appeared that way was really energy in motion? If something is solid, it has a shape and form and is fixed in terms of spatial coordinates such as height, width, and depth rather than fluid. In the world of space and time that human beings inhabit, we ordinarily think of physical objects as solid and ourselves as solid. ![]() ![]() These two levels of description, that of ‘strings’ and that of ‘quanta,’ convey to us a new understanding of ourselves as embodied beings… Like the tiny particles, we are made of energy capable of transforming us in more than one direction, and capable of changing our inner reality as a result. ![]() ![]() ![]() Schmidt's directions are clear and realistic, and she breaks down the writing process into manageable steps. ![]() Most important, this is not a book on how-to write a novel (although you'll find plenty of how-to writing tips in the book), rather it is about actually putting pen to paper and writing day in and day out until you reach your goal. The book is spiral bound, so that the pages will lay flat to more easily fill out all the worksheets and charts. You might want to buy a couple of copies so that you'll have a new copy on hand when you start your next novel - as you'll be wanting to use it over and over again. ![]() You'll use this book everyday and because you'll be writing continuously in the book. Rather than a book just to read, Book in a Month is a workbook filled with numerous charts and worksheets and exercises. Most important, by completing this draft you will not only have a solid draft to work from, but you'll also gain the undeniable knowledge that you can indeed write the novel you've always wanted! Granted, this will be a draft that will still need a lot of work, but it will have the basic form and shape of your completed novel. Rather, you'll have a complete draft of your novel. It is not the intention of this program that you have a complete, polished, and ready to publish novel at the end of the thirty days. This is a book that is designed to show you how to write a first draft of your novel, from beginning to end. Now before you start crying out that this is not possible, let me explain what this book does, and does not, do for you. This marvelous writer's reference provides a succinct and easy to follow plan that walks you through the steps of writing a novel in a mere thirty days! Reviewed by Herbert White - Posted July 28, 2012Ĭan you write a book in 30 days? Well I did, following the plan delineated in Victoria Lynn Schmidt's new book, Book in a Month: The Fool-Proof System for Writing a Novel in 30 Days. The Fool-Proof System for Writing a Novel in 30 Days Home | What's New? | Bookstore | Reviews | Articles So what are you waiting for? If you've been putting off your book project, let Book in a Month be your guide and find out just how much you can accomplish.Affiliate Disclosure: This site contains affiliate links, if you click on one of these links and make a purchase, we may receive a commission. Relax and have fun-you are, after all, doing something you love Keep track of the areas you want to revise without losing your momentum in the middle of your story Quickly outline your entire story so that you have a clear idea of how your plot and characters are going to develop before you start writingĭraft each act of your story by focusing on specific turning points Select a story topic that will continue to inspire you throughout the writing process Manage your time so that your writing life has room to flourish Set realistic goals and monitor your progress ![]() Using a combination of flexible weekly schedules, focused instruction, and detailed worksheets, author Victoria Schmidt leads you through a proven 30-day novel-writing system without the intimidation factor. If you make time to write and put away all of your excuses, could you stay on track and finish your novel in only a month? With a structured plan and a focused goal, yes, you can! ![]() 1/19/2023 0 Comments Using topaz denoise ai![]() Looking carefully at both images, you can see that the stacked image on the right looks cleaner than the other. How would DeNoise AI, uh, stack up against this tried-and-true process that night photographers and astronomers use? I used a stack of 15 photos for the comparison. I’ve done this for several years to reduce the noise. I did have several hot pixels, and it did not remove those, but everything else looked great. Looking at the comparison, you can once again see that DeNoise AI has significantly reduced the noise while keeping the details of the rock and stars intact. The 4-panel comparison in Topaz DeNoise AI, applying noise reduction to a single high ISO exposure.Ībove, I chose the Low Light from the 4-panel AI Model options. I realize that no one looks at their photos this way, but for the sake of comparison, it makes it easier to see. We’ll compare all the photos at 200% zoom. I use an app called Starry Landscape Stacker (Mac only) to combine the images. These random elements are rendered invisible in the final image. However, it removes random elements such as digital noise as well as elements that change from one exposure to the next, such as airplane trails. The final image shows the parts of the exposures that are consistent through each layer, like the stars. The stacking process is based on the median of all the stacked exposures. Stacking is when the photographer uses the same camera and tripod setup and the same settings and takes several photos in succession. Many people love to stack photos to reduce the camera’s digital noise. Topaz DeNoise AI, and a stacked photo vs. ![]() From the same camera and tripod setup, I wanted to compare two scenarios - a single exposure vs. It’s also a nearby place to photograph the night sky. After all, it’s the location of the epic slow-motion battle between Captain Kirk and the Gorn lizard-man. It’s a special place, particularly for “Star Trek” lovers. Vasquez Rocks is a state park north of Los Angeles. I decided to really challenge it even more at one of my favorite locations nearby, Vasquez Rocks in Agua Dulce. ![]() Its ability to reduce noise while keeping the stars sharp and intact was impressive. Previously, I had had used Topaz Labs DeNoise AI with a high ISO Milky Way image, which I wrote about here. ![]() |
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